꾸지뽕나무(Cudrania tricuspidata) 잎의 추출물이 대장암세포주에 미치는 영향
Methanol Extract of Leaves from Cudrania tricuspidata Effects in HT-29 Colorectal Adenocarcinoma
김미숙, 김인애, 고영종, 정진안, 김지은, 송병준, 임원봉, 임회선, 박지일, 김서연, 최홍란, 김옥준,
소속 상세정보
김미숙 ( Kim Mi-Sook ) - 전남보건환경연구원
김인애 ( Kim In-Ae ) - 전남대학교 치의학전문대학원 구강병리학교실 및 치의학연구소
고영종 ( Ko Young-Jong ) - 전남대학교 치의학전문대학원 구강병리학교실 및 치의학연구소
정진안 ( Jeong JIn An ) - 전남대학교 치의학전문대학원 구강병리학교실 및 치의학연구소
김지은 ( Kim JI-Eun ) - 전남대학교 치의학전문대학원 구강병리학교실 및 치의학연구소
송병준 ( Song Byung-Jun ) - 전남보건환경연구원
임원봉 ( Lim Won-Bong ) - 전남대학교 치과대학 구강병리학교실 및 치의학연구소
임회선 ( Lim Hoi-Soon ) - 전남대학교 치의학전문대학원 구강병리학교실 및 치의학연구소
박지일 ( Park Ji-Il ) - 광주보건대학교 치위생과
김서연 ( Kim Seo-Yune ) - 송원대학 치위생과
최홍란 ( Choi Hong-Ran ) - 전남대학교 치의학전문대학원 구강병리학교실 및 치의학연구소
김옥준 ( Kim Ok-Joon ) - 전남대학교 치의학전문대학원 구강병리학교실 및 치의학연구소
KMID : 0829220090330010019
Abstract
The phytochemicals of many plants suggest their potential use as dietary supplements in cancer chemoprevention and chemotherapy. In the present study, antitumor activity of Cudrania tricuspidata, a plant native to East Asia, was investigated. Cell growth inhibition of the extract on HT-29 colorectal adenocarcinoma using MTT colorimetric assay was determined. Apoptosis on HT-29 cells was performed by DNA fragmentation analysis. PGE2 release was measured by enzyme immunoassay, because PGE2 is a key protumorigenic prostanoid in many human cancers. For the ROS scavenging activity, ROS level was detected by laser scanning confocal microscope. It was found that methanol extract of leaves inhibits cell viability by inducing apoptosis as evidenced by DNA fragmentation. Stem bark decreases synthesis of PGE2, inflammatory mediator. Fruits exhibited pronounced ROS scavenging activity. Taken together, these results suggest that Cudrania tricuspidata exerts growth inhibition and anti-oxidation on HT-29 cells through apoptosis, ROS scavenging respectively that it may have anti-cancer properties.
키워드
Cudrania tricuspidata;Anti-inflammation;Anti-oxidation
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